************************************** ProAnWin - Protein Analyst for Windows ************************************** ver2.0 Program for analysis of protein sequences and structures, for studying the structure-activity relationships in protein families and design of protein-engineering experiments Copyright(c)1995-96 I.Pika, A.Frolov, V.Ivabisenko, A.Eroshkin The files required to run ProAnWin are distributed in the form of a single compressed file. Create a directory "PROANWIN" in your hard disk, for example, C. Copy the file to the directory, run the file from DOS prompt and answer Yes to all questions. To start the program run PROAWIN.EXE from windows. Program content: Directory: Main directory - program modules DATA - amino acid physico-chemical properties, manual, examples with input and output files ALIGNS - 50 aligned protein family sequences Program features: - Data input from main protein sequence formats (SWISS-PROT, PIR, FASTA, GCG); - Multiple protein sequences alignment (both automatic and manual); - Threading of multiple alignment onto known 3-dimensional structure; - Searches sites (in multiple protein alignment and protein 3D structure) influencing activity; - Analyses relationships between site structural characteristics and protein activities; - Analyses structural differences between proteins divided by functional, evolutionary or other criteria; - Investigates physico-chemical factors related with activity changes in a set of mutant proteins; - Helps to simulate protein-engineering experiments and to predict protein activity; - Provides possibility to analyze multiple physico-chemical factors; - Makes protein 3D pictures (mono and stereo) with sites highlighted; - The number of sequences is limited in this version.